LOCUS       pLV-[hsa-miR-380]        9115 bp    DNA     circular      3-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7036..7896
                     /label=Amp(R)
     promoter        8097..8637
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5641..5875
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6450..6905)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5947..6077
                     /label=SV40 pA
     misc_feature    6078..6291
                     /label=SV40 ori
     misc_feature    4962..5558
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3580
                     /label=intron
     CDS             3627..4952
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3370
                     /label=hsa-mir-380 precursor
BASE COUNT     2167 a      2372 c      2468 g      2108 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct ctcaatacta 
     3121 catcaggcct cagagggctt cctggaggtg atgctagtga gaggaaagag acaccggctc 
     3181 tgacctcagc cctctccaag gtacctgaaa agatggttga ccatagaaca tgcgctatct 
     3241 ctgtgtcgta tgtaatatgg tccacatctt ctcaatatca aattcagtca tagagggctt 
     3301 cccagagaga ggcaaagtca ccttcgtggg agacacactg atctggcttc agcgccattc 
     3361 cacagtactt gctagctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt 
     3421 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca 
     3481 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa 
     3541 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa aactacccct 
     3601 ctagagtcga gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca 
     3661 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 
     3721 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 
     3781 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 
     3841 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 
     3901 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 
     3961 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca 
     4021 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct 
     4081 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 
     4141 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 
     4201 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg 
     4261 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg 
     4321 acgagctgta caaggaccca ccggtcgcca ccatgaccga gtacaagccc acggtgcgcc 
     4381 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 
     4441 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 
     4501 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 
     4561 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 
     4621 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 
     4681 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 
     4741 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 
     4801 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 
     4861 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga 
     4921 cctggtgcat gacccgcaag cccggtgcct gagcggccgc gtcgacaatc aacctctgga 
     4981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 
     5041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 
     5101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 
     5161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 
     5221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 
     5281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 
     5341 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 
     5401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 
     5461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 
     5521 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 
     5581 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 
     5641 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 
     5701 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
     5761 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
     5821 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 
     5881 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 
     5941 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     6001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     6061 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa 
     6121 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     6181 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     6241 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 
     6301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 
     6361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 
     6421 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 
     6481 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     6541 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     6601 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     6661 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     6721 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     6781 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     6841 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 
     6901 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 
     6961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 
     7021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 
     7081 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 
     7141 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 
     7201 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 
     7261 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 
     7321 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 
     7381 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 
     7441 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 
     7501 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 
     7561 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 
     7621 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 
     7681 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 
     7741 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 
     7801 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 
     7861 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 
     7921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 
     7981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 
     8041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
     8101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 
     8161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 
     8221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 
     8281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 
     8341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
     8401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 
     8461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 
     8521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 
     8581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 
     8641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
     8701 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 
     8761 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 
     8821 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 
     8881 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 
     8941 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 
     9001 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 
     9061 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgc 
//
